Infection of eucaryotic cells by helper-independent recombinant adenoviruses: early region 1 is not obligatory for integration of viral DNA
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چکیده
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متن کاملInfluence of E1-Deleted Recombinant Adenoviruses on B7.1 and IL-2 Expression in C1498 Cells
Knowing that adenoviral vectors could initiate innate immunity, the ability of E1-deleted recombinant adenovirus (Ad-E1Δ) in induction of B7.1 and IL-2 molecules was studied. Methods: The expression of green fluorescent protein in C1498 cells following transfection of these cells with adenovirus green fluorescent protein vector confirmed the ability of adenovirus vectors in infecting the cells ...
متن کاملHelper-independent and AAV-ITR-independent chromosomal integration of double-stranded linear DNA vectors in mice.
Nonviral plasmid DNA is a promising vector for achieving ex vivo and in vivo gene transfer. However, transgene expression is usually transient, especially in dividing target cells due to loss of vector genomes. Here we describe the use of naked double-stranded (ds) linear DNA as a way to insert exogenous DNA sequences into chromosomes of mouse hepatocytes in vivo, without helper components such...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1984
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.50.2.606-614.1984